Download BLAST Software and Databases

BLAST+ executables

BLAST+ is a suite of command-line tools to run BLAST. For details, please see the BLAST+ user manual, the BLAST Help manual, the BLAST releases notes, and the article in BMC Bioinformatics (PubMed link). BLAST+ is the most current version of BLAST and is the only supported version.

Installers and source code are available from ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/ .

See our versioning policy.

The BLAST+ suite is the currently supported package. The older C toolkit executables are no longer supported.

Magic-BLAST

Magic-BLAST is a tool for mapping large next-generation RNA or DNA sequencing runs against a whole genome or transcriptome. Read more about Magic-BLAST at https://ncbi.github.io/magicblast.

Installers and source code are available from ftp://ftp.ncbi.nlm.nih.gov/blast/executables/magicblast/LATEST.

IgBLAST

IgBLAST facilitates the analysis of immunoglobulin and T cell receptor variable domain sequences. For details please see the documentation at https://ncbi.github.io/igblast/ and the article in Nucleic Acids Research (https://www.ncbi.nlm.nih.gov/pubmed/23671333).

Installers and source code are available from ftp://ftp.ncbi.nih.gov/blast/executables/igblast/release/LATEST.

SRPRISM

SRPRISM is a short read alignment tool that works with genomic sequences and handles alternative loci. For more information, see ftp://ftp.ncbi.nlm.nih.gov/pub/agarwala/srprism/README. A LINUX executable is available under ftp://ftp.ncbi.nlm.nih.gov/pub/agarwala/srprism

Databases

BLAST databases are updated daily and may be downloaded via FTP from ftp://ftp.ncbi.nlm.nih.gov/blast/db/. Database sets may be retrieved automatically with update_blastdb.pl, which is part of the BLAST+ suite. Please refer to the BLAST database documentation for more details.

The NCBI makes searchable collection of position-specific scoring matrices that can be used for sensitive protein and translated nucleotide searches. These are known as the Conserved Domain Database and can be searched with the RPSBLAST and RPSTBLASTN executables distributed with the BLAST+ package. The most comprehensive collection of CDD's is available at ftp://ftp.ncbi.nlm.nih.gov/pub/mmdb/cdd/little_endian/Cdd_NCBI_LE.tar.gz. Subsets of the comprehensive collection are available at ftp://ftp.ncbi.nlm.nih.gov/pub/mmdb/cdd/little_endian/. Documentation on these CDD collections is available at ftp://ftp.ncbi.nlm.nih.gov/pub/mmdb/cdd/README.